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DOI: 10.4049/jimmunol.0903879
¤ OpenAccess: Bronze
This work has “Bronze” OA status. This means it is free to read on the publisher landing page, but without any identifiable license.

LAG-3 Expression Defines a Subset of CD4+CD25highFoxp3+ Regulatory T Cells That Are Expanded at Tumor Sites

Chiara Camisaschi,Chiara Casati,Francesca Rini,Michela Perego,Annamaria De Filippo,Frédéric Triebel,Giorgio Parmiani,Filiberto Belli,Licia Rivoltini,Chiara Castelli

Expression (computer science)
Lag
Cell biology
2010
Abstract Human natural regulatory CD4+ T cells comprise 5–10% of peripheral CD4+T cells. They constitutively express the IL-2Rα−chain (CD25) and the nuclear transcription Foxp3. These cells are heterogeneous and contain discrete subsets with distinct phenotypes and functions. Studies in mice report that LAG-3 has a complex role in T cell homeostasis and is expressed in CD4+CD25+ T regulatory cells. In this study, we explored the expression of LAG-3 in human CD4+ T cells and found that LAG-3 identifies a discrete subset of CD4+CD25highFoxp3+ T cells. This CD4+CD25highFoxp3+LAG-3+ population is preferentially expanded in the PBMCs of patients with cancer, in lymphocytes of tumor-invaded lymph nodes and in lymphocytes infiltrating visceral metastasis. Ex vivo analysis showed that CD4+CD25highFoxp3+LAG-3+ T cells are functionally active cells that release the immunosuppressive cytokines IL-10 and TGF-β1, but not IL-2. An in vitro suppression assay using CD4+CD25highLAG-3+ T cells sorted from in vitro expanded CD4+CD25high regulatory T cells showed that this subset of cells is endowed with potent suppressor activity that requires cell-to-cell contact. Our data show that LAG-3 defines an active CD4+CD25highFoxp3+ regulatory T cell subset whose frequency is enhanced in the PBMCs of patients with cancer and is expanded at tumor sites.
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    LAG-3 Expression Defines a Subset of CD4+CD25highFoxp3+ Regulatory T Cells That Are Expanded at Tumor Sites” is a paper by Chiara Camisaschi Chiara Casati Francesca Rini Michela Perego Annamaria De Filippo Frédéric Triebel Giorgio Parmiani Filiberto Belli Licia Rivoltini Chiara Castelli published in 2010. It has an Open Access status of “bronze”. You can read and download a PDF Full Text of this paper here.