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Her-2/neu Overexpression Due to Polysomy 17 in Breast Cancer: Molecular Testing to Guide Therapeutic Options

Rashmita Sahoo,VenkataPradeep Babu,V Vidya Harini,Geeta V Patil,Gopal Krishna Dhondalay,Jayashree D Kulkarni,Ashwini Nargund,Smitha Rao,E Venkataswamy,B.S. Ajaikumar,Raghavendra Rao

Polysomy

Immunohistochemistry

Breast cancer

2011

Background: Fluorescent in situ hybridization (FISH) equivocal results for Her-2/neu still pose a diagnostic dilemma in oncology practice. In this study, we evaluate if Her-2/neu mRNA expression is an alternative to FISH for detecting Her-2/neu positivity. Patients and Methods: Archival paraffin blocks of 54 breast cancer patients were analyzed for Her-2/neu status using immunohistochemistry (IHC), FISH, and Her-2/neu gene expression using mRNA. Results: There was a 100% positive agreement and 64.7% negative agreement of Her-2/neu mRNA expression with respect to the reference standard (FISH), with the kappa value for agreement being 0.36. mRNA levels correlated positively and strongly with FISH ratio and IHC positivity. For Her-2/neu mRNA expression, Her-2/neu copy number was a significant predictor indicating that mRNA expression is independent of polysomy status. Conclusions: Her-2/neu mRNA expression may help tide over ambiguity posed by polysomy and FISH equivocal samples.

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“Her-2/neu Overexpression Due to Polysomy 17 in Breast Cancer: Molecular Testing to Guide Therapeutic Options” is a paper by Rashmita Sahoo VenkataPradeep Babu V Vidya Harini Geeta V Patil Gopal Krishna Dhondalay Jayashree D Kulkarni Ashwini Nargund Smitha Rao E Venkataswamy B.S. Ajaikumar Raghavendra Rao published in 2011. It has an Open Access status of “closed”. You can read and download a PDF Full Text of this paper here.