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DOI: 10.1126/science.1259425
¤ OpenAccess: Bronze
This work has “Bronze” OA status. This means it is free to read on the publisher landing page, but without any identifiable license.
An interactive reference framework for modeling a dynamic immune system
Matthew H. Spitzer,Pier Federico Gherardini,Gabriela K. Fragiadakis,Nupur Bhattacharya,Robert Yuan,Andrew Hotson,Rachel Finck,Yaron Carmi,Eli R. Zunder,Wendy J. Fantl,Sean C. Bendall,Edgar G. Engleman,Garry P. Nolan
Mass cytometry
Immune system
Biology
2015
Immune cells function in an interacting hierarchy that coordinates the activities of various cell types according to genetic and environmental contexts. We developed graphical approaches to construct an extensible immune reference map from mass cytometry data of cells from different organs, incorporating landmark cell populations as flags on the map to compare cells from distinct samples. The maps recapitulated canonical cellular phenotypes and revealed reproducible, tissue-specific deviations. The approach revealed influences of genetic variation and circadian rhythms on immune system structure, enabled direct comparisons of murine and human blood cell phenotypes, and even enabled archival fluorescence-based flow cytometry data to be mapped onto the reference framework. This foundational reference map provides a working definition of systemic immune organization to which new data can be integrated to reveal deviations driven by genetics, environment, or pathology.
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“An interactive reference framework for modeling a dynamic immune system” is a paper by Matthew H. Spitzer Pier Federico Gherardini Gabriela K. Fragiadakis Nupur Bhattacharya Robert Yuan Andrew Hotson Rachel Finck Yaron Carmi Eli R. Zunder Wendy J. Fantl Sean C. Bendall Edgar G. Engleman Garry P. Nolan published in 2015. It has an Open Access status of “bronze”. You can read and download a PDF Full Text of this paper here.