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A STUDY OF THE MUSCARINIC RECEPTOR BY GEL ELECTROPHORESIS

N. J. M. Birdsall,A. S. V. Burgen,Edward C. Hulme

Muscarinic acetylcholine receptor

Proteolysis

Gel electrophoresis

1979

1 Muscarinic receptors from the brain of rat, guinea-pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([3H]-PrBCM). 2 After solubilisation and sodium dodecyl sulphate (SDS)-polycarylamide gel electrophoresis a single radiolabelled peak was seen which was completely suppressed when labelling was carried out in the presence of 10(-6) M atropine. The results suggest the presence of a single polypeptide chain with a molecular weight of similar to or approximately 80,000. 3 In the case of intestinal smooth muscle multiple labelled peaks were found. Precautions against proteolysis led to the identification of a major peak with a molecular weight in the region of 80,000.

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“A STUDY OF THE MUSCARINIC RECEPTOR BY GEL ELECTROPHORESIS” is a paper by N. J. M. Birdsall A. S. V. Burgen Edward C. Hulme published in 1979. It has an Open Access status of “green”. You can read and download a PDF Full Text of this paper here.