Sequencing thousands of single-cell genomes with combinatorial indexing

Single-cell genome sequencing has proven valuable for the detection of somatic variation, particularly in the context of tumor evolution.Current technologies suffer from high library construction costs which restrict the number of cells that can be assessed and thus impose limitations on the ability to measure heterogeneity within a tissue.Here, we present Single cell Combinatorial Indexed Sequencing \(SCI-seq) as a means of simultaneously generating thousands of low-pass single cell libraries for somatic copy number variant detection.