Cytokine Expression After Vaginal Distention of Different Durations in Virgin Sprague-Dawley Rats

No AccessJournal of UrologyInvestigative Urology1 Aug 2008Cytokine Expression After Vaginal Distention of Different Durations in Virgin Sprague-Dawley Ratsis companion ofLaparoscopic Ureteroneocystostomy and Psoas Hitch for Post-Hysterectomy Ureterovaginal Fistula Hadley M. Wood, Mei Kuang, Lynn Woo, Adonis Hijaz, Robert S. Butler, Marc Penn, Raymond Rackley, and Margot S. Damaser Hadley M. WoodHadley M. Wood Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio More articles by this author , Mei KuangMei Kuang Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio Department of Cancer Biology, Cleveland Clinic, Cleveland, Ohio More articles by this author , Lynn WooLynn Woo Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio More articles by this author , Adonis HijazAdonis Hijaz Department of Urology, Case Western Reserve University, Cleveland, Ohio Financial interest and/or other relationship with Pfizer. More articles by this author , Robert S. ButlerRobert S. Butler Department of Quantitative Health Sciences, Cleveland Clinic, Cleveland, Ohio More articles by this author , Marc PennMarc Penn Department of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio Department of Cardiovascular Medicine and Stem Cell Biology and Regenerative Medicine, Cleveland Clinic, Cleveland, Ohio More articles by this author , Raymond RackleyRaymond Rackley Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio Department of Cancer Biology, Cleveland Clinic, Cleveland, Ohio Financial interest and/or other relationship with Schering, Pfizer, Novartis, Boston Scientific and NDI Medical. More articles by this author , and Margot S. DamaserMargot S. Damaser Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio Department of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio Research Service, Louis Stokes Cleveland Veterans Affairs Medical Center, Cleveland, Ohio More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2008.03.182AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We investigated the effect of the duration of vaginal distention on the differential expression of stem cell homing, tissue repair cytokines and cytokine receptors to identify the factors most important for recovery from injury. Materials and Methods: A total of 20, 10-week-old virgin Sprague-Dawley rats were divided into 4 groups, including 1, 4 and 6-hour vaginal distention, and anesthetized sham operation. The vagina, bladder, urethra and rectum were harvested immediately after vaginal distention. Real-time polymerase chain reaction was used to determine the relative expression of cytokines and receptors of interest. Mixed models analysis was used to determine associations between expression levels and vaginal distention duration. Results: Positive associations between vaginal distention duration and the urethral expression level were found for 1 of the receptors of monocyte chemotactic protein-3, CCR1 (p = 0.0001) as well as for monocyte chemotactic protein-3 (p = 0.025), CCR5 (p = 0.032) and hypoxia inducible factor-1α (p = 0.023). A positive relationship between vaginal distention duration and monocyte chemotactic protein-3 expression was also observed in rectal tissue (p = 0.035). Urethral expression of CCR2, another receptor for monocyte chemotactic protein-3, approached significance (p = 0.066). An inverse relationship between vaginal distention duration and interleukin-8 expression was found in the bladder (p = 0.0008). No association was noted between vaginal distention duration and the expression of stromal derived factor-1, CXCR4, CCR3 and vascular endothelial growth factor in any pelvic organs. Conclusions: These data support a relationship between vaginal distention duration and the subsequent expression of monocyte chemotactic protein-3 and 1 of its associated receptors, CCR1, in the urethra immediately following vaginal distention. The increase in hypoxia-inducible factor1α expression in the urethra with prolonged vaginal distention suggests a limited role of tissue ischemia in the immediate response of pelvic organs to vaginal distention. References 1 : Parity, mode of delivery, and pelvic floor disorders. Obstet Gynecol2006; 107: 1253. Google Scholar 2 : The effect of simulated birth trauma on the urinary continence mechanism in the rat. Urology1998; 52: 143. Google Scholar 3 : Increased duration of simulated childbirth injuries results in increased time to recovery. Am J Physiol Regul Integr Comp Physiol2007; 292: R1738. Google Scholar 4 : Overexpression of stem cell homing cytokines in rat pelvic organs following vaginal distension. J Urol2007; 177: 1568. Link, Google Scholar 5 : Effects of vaginal distension on urethral anatomy and function. BJU Int2002; 90: 403. Google Scholar 6 : Monocyte chemotactic protein-3 is a myocardial mesenchymal stem cell homing factor. Stem Cells2007; 25: 245. Google Scholar 7 : Cellular and developmental control of O2 homeostasis by hypoxia-inducible factor 1 alpha. Genes Dev1998; 12: 149. Google Scholar 8 : Effect of stromal-cell-derived factor 1 on stem-cell homing and tissue regeneration in ischaemic cardiomyopathy. Lancet2003; 362: 697. Google Scholar 9 : Chemokines and chemokine receptors in leukocyte trafficking. Am J Physiol Regul Integr Comp Physiolo2002; 283: R7. Google Scholar 10 : Interleukin-8 is a mediator of the final cervical ripening in humans. Eur J Obstet Gynecol1997; 74: 89. Google Scholar 11 : Expression of interleukin-8 receptors in the gestational tissues before and after initiation of labor: immunohistochemical study. Acta Obstet Gynecol Scand1996; 75: 790. Google Scholar 12 : Monocyte chemoattractant protein-3 (MCP-3) interacts with multiple leukocyte receptors: binding and signaling of MCP-3 through shared as well as unique receptors on monocytes and neutrophils. Eur J Immunol1995; 25: 2612. Google Scholar 13 : Impaired host defense, hematopoiesis, granulomatous inflammation and type 1-type 2 cytokine balance in mice lacking CC chemokine receptor I. J Exp Med1997; 185: 1959. Google Scholar 14 : The chemokine receptor CCR1 is strongly upregulated after skin injury but dispensable for wound healing. Wound Repair Regen2004; 12: 193. Google Scholar 15 : Purification of a human monocyte-derived neurotrophic chemotactic factor that has peptide sequence similarity to other host defense cytokines. Proc Natl Acad Sci U S A1987; 84: 9233. Google Scholar 16 : Combined urinary and faecal incontinence. Int Urogynecol J Pelvic Floor Dysfunct2005; 16: 321. Google Scholar 17 : Atlas of Human Anatomy. New York: WB Saunders Elsevier2006. Google Scholar 18 : Reduced blood flow and hypoxia of urogenital organs of the female rat due to vaginal distension. J Appl Physiol2005; 98: 1884. Google Scholar 19 : Gene expression profiling of cervical tissue during physiological cervical effacement. Obstet Gynecol2005; 105: 91. Google Scholar 20 : Monocyte chemotactic protein-2 and -3 in amniotic fluid: relationship to microbial invasion of the amniotic cavity, intra-amniotic inflammation and preterm delivery. Acta Obstet Gynecol Scand2005; 84: 566. Google Scholar 21 : Cyclic mechanical stretch augments both interleukin-8 and monocyte chemotactic protein-3 production in the cultured human uterine cervical fibroblast cells. Mol Human Reprod2004; 10: 573. Google Scholar © 2008 by American Urological AssociationFiguresReferencesRelatedDetailsCited bySumino Y, Yoshikawa S, Mimata H and Yoshimura N (2013) Therapeutic Effects of IGF-1 on Stress Urinary Incontinence in Rats with Simulated Childbirth TraumaJournal of Urology, VOL. 191, NO. 2, (529-538), Online publication date: 1-Feb-2014.Lenis A, Kuang M, Woo L, Hijaz A, Penn M, Butler R, Rackley R, Damaser M and Wood H (2012) Impact of Parturition on Chemokine Homing Factor Expression in the Vaginal Distention Model of Stress Urinary IncontinenceJournal of Urology, VOL. 189, NO. 4, (1588-1594), Online publication date: 1-Apr-2013.Related articlesJournal of Urology12 Jun 2008Laparoscopic Ureteroneocystostomy and Psoas Hitch for Post-Hysterectomy Ureterovaginal Fistula Volume 180Issue 2August 2008Page: 753-759 Advertisement Copyright & Permissions© 2008 by American Urological AssociationKeywordsurinary incontinencecytokinesratsSprague-DawleyvaginaparturitionstressAcknowledgmentsDavid Sypert provided technical assistance with the figures. This project was performed at the Lerner Research Institute, Cleveland Clinic. Primers/probe sets (SDF-1, CXCR-4, CCR-1, CCR-2, VEGF, CCR-3 and CCR-5) for TaqMan real-time PCR were obtained from Applied Biosystems, Inc.MetricsAuthor Information Hadley M. Wood Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio More articles by this author Mei Kuang Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio Department of Cancer Biology, Cleveland Clinic, Cleveland, Ohio More articles by this author Lynn Woo Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio More articles by this author Adonis Hijaz Department of Urology, Case Western Reserve University, Cleveland, Ohio Financial interest and/or other relationship with Pfizer. More articles by this author Robert S. Butler Department of Quantitative Health Sciences, Cleveland Clinic, Cleveland, Ohio More articles by this author Marc Penn Department of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio Department of Cardiovascular Medicine and Stem Cell Biology and Regenerative Medicine, Cleveland Clinic, Cleveland, Ohio More articles by this author Raymond Rackley Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio Department of Cancer Biology, Cleveland Clinic, Cleveland, Ohio Financial interest and/or other relationship with Schering, Pfizer, Novartis, Boston Scientific and NDI Medical. More articles by this author Margot S. Damaser Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio Department of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio Research Service, Louis Stokes Cleveland Veterans Affairs Medical Center, Cleveland, Ohio More articles by this author Expand All Advertisement PDF downloadLoading ...