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DOI: 10.3390/cells10082040
¤ OpenAccess: Gold
This work has “Gold” OA status. This means it is published in an Open Access journal that is indexed by the DOAJ.

High Glycolytic Activity Enhances Stem Cell Reprogramming of Fahd1-KO Mouse Embryonic Fibroblasts

Ahmad Salti,Solmaz Etemad,Marta Suarez Cubero,Eva Albertini,Beata Kovacs-Szalka,Magdalena Holzknecht,Elia Cappuccio,Maria Cavinato,Frank Edenhofer,Pidder Jansen Dürr

Reprogramming
Induced pluripotent stem cell
Cell biology
2021
Mitochondria play a key role in metabolic transitions involved in the reprogramming of somatic cells into induced pluripotent stem cells (iPSCs), but the underlying molecular mechanisms remain largely unexplored. To obtain new insight into the mechanisms of cellular reprogramming, we studied the role of FAH domain-containing protein 1 (FAHD1) in the reprogramming of murine embryonic fibroblasts (MEFs) into iPSCs and their subsequent differentiation into neuronal cells. MEFs from wild type (WT) and Fahd1-knock-out (KO) mice were reprogrammed into iPSCs and characterized for alterations in metabolic parameters and the expression of marker genes indicating mitochondrial biogenesis. Fahd1-KO MEFs showed a higher reprogramming efficiency accompanied by a significant increase in glycolytic activity as compared to WT. We also observed a strong increase of mitochondrial DNA copy number and expression of biogenesis marker genes in Fahd1-KO iPSCs relative to WT. Neuronal differentiation of iPSCs was accompanied by increased expression of mitochondrial biogenesis genes in both WT and Fahd1-KO neurons with higher expression in Fahd1-KO neurons. Together these observations establish a role of FAHD1 as a potential negative regulator of reprogramming and add additional insight into mechanisms by which FAHD1 modulates mitochondrial functions.
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    High Glycolytic Activity Enhances Stem Cell Reprogramming of Fahd1-KO Mouse Embryonic Fibroblasts” is a paper by Ahmad Salti Solmaz Etemad Marta Suarez Cubero Eva Albertini Beata Kovacs-Szalka Magdalena Holzknecht Elia Cappuccio Maria Cavinato Frank Edenhofer Pidder Jansen Dürr published in 2021. It has an Open Access status of “gold”. You can read and download a PDF Full Text of this paper here.