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DOI: 10.1080/00021369.1979.10863679
OpenAccess: Closed
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Sensitive and Simple Methods for Determination of<scp>l</scp>-Lysine with<scp>l</scp>-Lysine<i>α</i>-Oxidase

Hitoshi Kusakabe,Keiichi Kodama,Akira Kuninaka,Hiroshi Yoshino,Kenji Soda

Lysine
Chemistry
Oxidative deamination
1979
l-Lysine could be determined satisfactorily with a new fungal enzyme, l-Iysine α-oxidase (EC 1.4.3). The method consists of the oxidative deamination of l-Iysine with l-lysine α-oxidase and the spectrophotometric determination of one of the reaction products: α-keto-ε-aminocaproate, its intramolecular dehydrated form, Δ1-piperideine-2-carboxylate or hydrogen peroxide. The method on the basis of the color reaction of hydrogen peroxide formed from l-lysine with 4-aminoantipyrine and phenol in the presence of peroxidase was most sensitive and simple. The method could be used for the direct assay of l-lysine levels in serums from several animals without pretreatments.
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    Sensitive and Simple Methods for Determination of<scp>l</scp>-Lysine with<scp>l</scp>-Lysine<i>α</i>-Oxidase” is a paper by Hitoshi Kusakabe Keiichi Kodama Akira Kuninaka Hiroshi Yoshino Kenji Soda published in 1979. It has an Open Access status of “closed”. You can read and download a PDF Full Text of this paper here.