Molecular biology (Page 1)

Generalized lacZ expression with the ROSA26 Cre reporter strain

In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector

Function and Activation of NF-kappaB in the Immune System

Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma

Extension of Life-Span by Introduction of Telomerase into Normal Human Cells

Lanthanum Chloride Inhibits LPS Mediated Expressions of Pro-Inflammatory Cytokines and Adhesion Molecules in HUVECs: Involvement of NF-κB-Jmjd3 Signaling

A simple and very efficient method for generating cDNA libraries

Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5

Cloning of a novel receptor expressed in rat prostate and ovary.

The Release of Cytochrome c from Mitochondria: A Primary Site for Bcl-2 Regulation of Apoptosis

Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.

A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry

Identification of c- <i>MYC</i> as a Target of the APC Pathway

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

Caspases: the executioners of apoptosis

HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS

Real-time quantification of microRNAs by stem-loop RT-PCR

Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing

Comprehensive molecular profiling of lung adenocarcinoma

Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

Gain-of-Function Mutations of c- <i>kit</i> in Human Gastrointestinal Stromal Tumors

High-efficiency transformation of mammalian cells by plasmid DNA.

Dystrophin: The protein product of the duchenne muscular dystrophy locus

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

A rapid method for the isolation of functional thymus-derived murine lymphocytes

A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation

Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species

Chromosome preparations of leukocytes cultured from human peripheral blood

Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity

EGF receptor gene mutations are common in lung cancers from “never smokers” and are associated with sensitivity of tumors to gefitinib and erlotinib

HIV-1 Entry Cofactor: Functional cDNA Cloning of a Seven-Transmembrane, G Protein-Coupled Receptor

A New Consistent Chromosomal Abnormality in Chronic Myelogenous Leukaemia identified by Quinacrine Fluorescence and Giemsa Staining

Generation of germline-competent induced pluripotent stem cells

Osf2/Cbfa1: A Transcriptional Activator of Osteoblast Differentiation

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

The Ki-67 protein: From the known and the unknown

An Efficient mRNA-Dependent Translation System from Reticulocyte Lysates

A membrane-filter technique for the detection of complementary DNA

Molecular Beacons: Probes that Fluoresce upon Hybridization

Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes

Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis

CD14, a Receptor for Complexes of Lipopolysaccharide (LPS) and LPS Binding Protein

MicroRNA genes are transcribed by RNA polymerase II

Molecular characterization of mitochondrial apoptosis-inducing factor

Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice

A complementation analysis of the restriction and modification of DNA in Escherichia coli

Une micro-méthode de l’immuno-électrophorèse

Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.

Novel Regulators of Bone Formation: Molecular Clones and Activities

Mutation in blood coagulation factor V associated with resistance to activated protein C

Maturation of the head of bacteriophage T4

Targeted mutation of the DNA methyltransferase gene results in embryonic lethality

Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1.

Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase

Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death

Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes.

Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

Gene Action in the X-chromosome of the Mouse (Mus musculus L.)

Direct Gene Transfer into Mouse Muscle in Vivo

Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations

Mammalian microRNAs predominantly act to decrease target mRNA levels

An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels.

Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface

Phenotypic Analysis of Antigen-Specific T Lymphocytes

Particle-size Analysis

β-Catenin regulates expression of cyclin D1 in colon carcinoma cells

An Nrf2/Small Maf Heterodimer Mediates the Induction of Phase II Detoxifying Enzyme Genes through Antioxidant Response Elements

Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction

Bid, a Bcl2 Interacting Protein, Mediates Cytochrome c Release from Mitochondria in Response to Activation of Cell Surface Death Receptors

Silver staining of proteins in polyacrylamide gels

Synthesis of Proteins by Native Chemical Ligation

Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes

Cytokine-mediated regulation of CXCR4 expression in human neutrophils

Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.

CpG motifs in bacterial DNA trigger direct B-cell activation

A simple method for clinical assay of superoxide dismutase.

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

The TNF and TNF Receptor Superfamilies

Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67.

PD 098059 Is a Specific Inhibitor of the Activation of Mitogen-activated Protein Kinase Kinase in Vitro and in Vivo

Identification and expression cloning of a leptin receptor, OB-R

<i>LMO2</i> -Associated Clonal T Cell Proliferation in Two Patients after Gene Therapy for SCID-X1

RAS Is Regulated by the let-7 MicroRNA Family

Circular RNAs are abundant, conserved, and associated with ALU repeats

Structure of the human class I histocompatibility antigen, HLA-A2

Histone Demethylation Mediated by the Nuclear Amine Oxidase Homolog LSD1

<i>miR-15</i> and <i>miR-16</i> induce apoptosis by targeting BCL2

The 3′-Terminal Sequence of <i>Escherichia coli</i> 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites

Inhibition of Poly(ADP-Ribose) Polymerase in Tumors from<i>BRCA</i>Mutation Carriers

Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs

A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels

Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes

Smac, a Mitochondrial Protein that Promotes Cytochrome c–Dependent Caspase Activation by Eliminating IAP Inhibition

Identification of a gene (FMR-1) containing a CGG repeat coincident with a breakpoint cluster region exhibiting length variation in fragile X syndrome

A Novel Transcription Factor, T-bet, Directs Th1 Lineage Commitment

Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes).

Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data